Device

Part:BBa_K2321005

Designed by: Xianghui Zhao   Group: iGEM17_SDU_CHINA   (2017-10-24)


tetR repressible GFP device


tetR repressible promoter is like a switch, which determines the expression of GFP. When there was tetracycline, tetR would combine with tetracycline, so tetR can not repress the promoter anymore. This would start the transcription of GFP (as a reporter), which means GFP coding sequence is under the regulation of tetR repressible promoter. So when tetracycline came into cells, tetR is inhibited, and there would be green visible. In another word, tetR repression is inhibited by the addition of tetracycline or its analog.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 719


[edit]
Categories
Parameters
None